General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter CHO-K1 rCHRM4 β-Arrestin Cell Line, 2 vials. (93-0870C2)
The PathHunter rCHRM4 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. acetylcholine) based activation of rCHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rCHRM4 and EA-tagged β-Arrestin-2. Activation of rCHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Quantitative Genomic DNA from; Aspergillus flavus; Strain: SN 3
Quantitative Genomic DNA from Asperigillus flavus strain SN 3 can be used for assay development, verification, and validation as well as monitoring of day-to-day test variation and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine fungal load.
Eurofins Discoverx cAMP Hunter CHO-K1 HRH3 Gi Cell Line, 2 vials. (95-0103C2)
The cAMP Hunter HRH3 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. histamine) based activation of HRH3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HRH3, a naturally Gi/Go coupled GPCR. Upon activation of HRH3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Saccharolobus solfataricus; P2; genomic DNA
Genomic DNA isolated from Saccharolobus solfataricus strain DSM 1617 [P2] (ATCC 35092). This product can be used in PCR and other molecular biology applications.
Eurofins Discoverx PathHunter ADCYAP1R1 Total Internalization U2OS Cell Line, 2 vials. (93-0885C3)
The PathHunter ADCYAP1R1 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of ADCYAP1R1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged ADCYAP1R1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the ADCYAP1R1 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC Aspergillus clavatus; Kral; genomic DNA
Genomic DNA isolated from Aspergillus clavatus QM 1276. This fungal strain is also available as ATCC Catalog No.: 1007™.
Eurofins Discoverx PathHunter OPRM1 Total GPCR Internalization U2OS Cell Line, 2 vials. (93-0745C3)
The PathHunter OPRM1 Total GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of OPRM1 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged OPRM1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRM1 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx PathHunter CHO-K1 cyno MRGPRX2 β-Arrestin Cell Line, 2 vials. (93-1134C2)
The PathHunter cyno MRGPRX2 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. PAMP-20) based activation of cyno MRGPRX2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged cyno MRGPRX2 and EA-tagged β-Arrestin-2. Activation of cyno MRGPRX2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Cynomolgus monkey version of the protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx ChemiScreen ETA Endothelin Receptor Stable Cell Line, 2 vials. (HTS070C)
The ChemiScreen ETA Endothelin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. endothelin) based activation of EDNRA GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses EDNRA alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of EDNRA, the resulting calcium activity can be fluorescently assayed. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Influenza A virus (H1N1); A/Virginia/ATCC3/2009; TC isolate
Influenza A virus (H1N1) strain A/Virginia/ATCC3/2009 is propagated in MDCK (NBL-2) cells (ATCC CCL-34). This strain was isolated in 2009 from a nasopharyngeal specimen from a patient positive for Influenza A in Virginia. It has applications in respiratory research and influenza research.
Eurofins Discoverx ChemiScreen V1B Vasopressin Receptor Membrane Preparation, 1 vial. (HTS136M)
The ChemiScreen V1B Vasopressin Receptor Membrane Preparation is derived from proprietary stable recombinant cells to ensure high levels of AVPR1B surface expression. ChemiScreen membrane preps can be used to screen for ligand binding affinities using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1 mL of 1 mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. For detailed product information, visit www.discoverx.com/membranepreps.
Cell Applications Inc Human Mammary Epithelial Cell Total Kit
Human Mammary Epithelial Cell Total Kit(830-05a) Cryopreserved (HMEpC) 1 vial, adult(815-500) Mammary Epithelial Growth Media 500ml(090K) Subculture Reagent Kit
ATCC UM-Chor5D; Chordoma; Human (Homo sapiens)
There is a Permit requirement: License form needed to be completed Material Transfer Agreement Addendum for Screening Application
The forms are available here: https://www.atcc.org/support/order-support/permits-and-restrictions
Amsbio LLC TLR3/NF-kB Leeporter™ Luciferase Reporter-HEK293 Cell Line
TLR3/NF-kB Leeporter™ Luciferase Reporter-HEK293 Cell Line
Biochain Institute Inc Frozen Tissue Section - Human Adult Normal: Heart: Pericardium, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.
